Molecular identification of fungal infections in Children with Intermittent Peritoneal dialysis

الملخص

Background: Fungi is a major cause of peritonitis infections and it is very important to have a reliable test to detect these bacteria in peritoneal fluid by molecular methods . Aims of the study: Detection of the most common Fungi in peritoneal fluid from pediatric patients with acute and chronic renal failure who undergo peritoneal dialysis, through molecular diagnostic methods. Methods: One hundred clinical peritoneal fluid isolates were submitted to molecular methods (Polymerase chain reaction (PCR) and DNA sequencing) for detection of fungi. Results: By molecular methods according to PCR technique, the following Fugi were identified: C. tropicalis 6 (8.2%), C. albicanis 3 (4.1%), C. parpsilosis 2 (2.7%) and C. glebrata 1 (1.4%). According to DNA sequencing technique, the following fungi were identified: C. tropicalis 8 (11.0%), C. albicanis 5 (6.8%), C. parpsilosis 1 (1.4%), Penicillium chrysogenum 10 (13.7%), Aspergillus fumigatus 12 (16.4%), Chaetomium globosum 5 (6.8%) , Neurospora spp. 5(6.8%), Trichoderma spp. 3 (4.1%), Clavispora lusitaniae 8 (11.0%) and Rhodotorula mucilaginosa 6 (8.2%) Conclusion: The most common fungi were the best.pathogene responsible for peritonitis were C. tropicalis, C. albicanis and Aspergillus fumigates. Key words: peritonitis, Molecular detection of fungal peritonitis, peritoneal dialysis infections