Klebsiella pneumoniae in Hospital Acquired and Community Acquired Urinary Tract Infections in an Iraqi Cohort: Frequency, Antibiotic Susceptibility and the Percentage of bla KPC Resistance Gene

الملخص

Background: Klebsiella pneumoniae (K. pneumoniae) is an important pathogen in hospital and community-acquired urinary tract infections (HAUTI and CAUTI). It is showing a high resistance towards many antibioticsObjective: This study aims to estimate the percentage of K. pneumoniae in HAUTIs and CAUTIs, antibiotic resistance, extended-spectrum beta lactamase (ESBL), and detect the beta-lactamase K. pneumoniae carbapenemases (bla KPC) resistance gene and presence of a mutation in this gene. Methods: A total of 200 urine samples were collected randomly; K. pneumoniae was cultured and isolated on MacConkey agar, antibiotic sensitivity testing, and ESBL detection were performed by Vitek 2 system, then DNA was extracted, polymerase chain reaction and sequencing for KPC gene were done.Results: There were 7% (14 isolates) of K. pneumoniae (50%) HAUTI and (50%) CAUTI. ESBL was positive in 3 isolates (21.43%). The percentage of positive bla KPC gene was 42.8%. KPC gene sequencing showed a high rate of mutation in positive isolates reaching to 50 different nucleotides. Conclusion: This work has increased the knowledge of K. pneumoniae causing HAUTI and CAUTI in Iraqi patients.Keywords: Klebsiella pneumoniae, hospital-acquired urinary tract infection, community-acquired urinary tract infection, ESBL, bla KPC gene.Citation: Nasser HH, Abdulrahman TR, Malik AS. Klebsiella pneumoniae in hospital-acquired and community-acquired urinary tract infections in an Iraqi cohort: Frequency, antibiotic susceptibility and the percentage of bla KPC resistance gene. Iraqi JMS. 2022; 20(1): 121-131. doi: 10.22578/IJMS.20.1.16