A Molecular approach to detecting type B Coxsackie virus in a cohort Iraqi patient with different type of abortion

الملخص

Background The infection can present in newborns in different ways, ranging from no noticeable symptoms to more serious conditions like meningoencephalitis, myocarditis, and disseminated sepsis. Methods A cross-sectional study was conducted from November 2022 to June 2023. The study included 100 pregnant women with gestational ages ranging from 0 to 16 weeks, aged between 15 and 40 years. Each participant completed a standardized questionnaire. Afterward, placental tissue samples were collected from women who underwent elective termination of pregnancy. These samples were then analyzed for the presence of Coxsackievirus B (CVB) genome using a molecular biology approach. The detection process involved RNA extraction, cDNA, and qRT-PCR. Results A total of 100 women who had abortions were included in this study. Out of these, 88 had missed abortions, 10 had incomplete abortions …